手术切除小细胞肺癌P53蛋白表达及意义

Expression of P53 Protein in Resected Small Cell Lung Cancer and Its Significance

目的研究手术切除小细胞肺癌（ＳＣＬＣ）Ｐ５３蛋白表达，探讨其临床意义。方法取根治术后＞５年，术前未放疗、化疗标本３２例；石蜡切片，ＡＢＣ染色。结果Ｐ５３蛋白表达总阳性率８１．３％（２６／３２）；Ｐ５３蛋白表达与性别、吸烟、ＰＴＮＭ分期、淋巴结转移、原发病灶大小相关不显著（Ｐ＞０．０５）。术后２年内死亡组Ｐ５３阳性率１００％（１４／１４），＞５年死亡组４２．９％（３／７）（Ｐ＜０．０１）。前者死亡风险比后者高２倍。脑转移者Ｐ５３阳性率８５．７％（６／７）。结论Ｐ５３蛋白在ＳＣＬＣ中有高表达，它与ＳＣＬＣ术后的近期死亡及脑转移有关。

Objective To study the expression of P53 protein in resected pathologically small cell lung cancer (SCLC) and to explore its clinical significance. Methods Thirty two samples were taken according to standard over 5 years after radical surgical operation and without radiotherapy and chemotherapy before the operation, with paraffin embedded specimens and immunohistochemical ABC methods. Results The total positive expression rate of P53 protein was found to be 81.3%(26/32). The correlation between the expression rate of P53 protein and sex, smoking, PTNM stage, lymph node metastasis and primary cancer focus had no statistics significance ( P>0.05) .With postoperative patients both survival less than 2 years and more than 5 years, the frequency of P53 expression reached 100% (14/14) and 42.9% (3/7) ( P<0.01) respectively, but the relative risk of death was high about 2 times for former to later. The frequency showed 85.7% (6/7) in the patients with the brain metastasis. Conclusion There was obvious overexpression of P53 protein in SCLC. It is related to the recent death and brain metastases after surgical treatment. P53 protein is a good index to estimate postoperative prognosis of SCLC.