摘要
目的:探讨胶质细胞源性神经生长因子(GDNF)基因转染培养的视网膜神经细胞的可能性及其表达情况。方法:培养的SD大鼠视网膜神经细胞应用脂质体法转染报道基因(pcDNA3-LacZ)和pcDNA3-GDNF,应用RT-PCR及SDS-PAGE电泳检测其表达情况。结果:pcDNA3-LacZ可转染到视网膜神经细胞。应用RT-PCR可在转染的培养细胞中检测到GDNF转录水平的表达,并可通过SDS-PAGE电泳观察到32KD的表达产物。结论:pcDNA3-LacZ可以作为视网膜神经细胞转基因成功与否的判别指标。pcDNA3-GDNF可转染培养的SD视网膜神经细胞,并可检测到其表达,为青光眼的视功能损害防治提供了一种新的可能。眼科学报1998;14:57—60。
Purpose:To investigate the possibility of gene transfer into retina neural cells. Methods :pcDNA3-LacZ and pcDNA3-GDNF were transferred into rat retinal neural cells with cationic lipofectin. The gene transfer and expression of GDNF were detected by means of RT-PCR and SDS-PAGE.
Results: pcDNA3-LacZ and pcDNA3-GDNF could be transferred into retinal neural cells and the expressive product of GDNF gene, a 32KD protein band, was detected. Conclusion: The preliminary results suggest that LacZ gene could be a marker to identify gene transfer into the target tissue and cells; GDNF gene could be transferred into cultured retinal neural cells of SD rats, and express 32KD protein. Eye Science 1998; 14:57-60.
出处
《眼科学报》
1998年第2期57-60,共4页
Eye Science
基金
国家自然科学基金(39700153
39700789)
中国博士后研究基金
广东省博士后研究基金
