眼部淋巴组织增生性病变PCNA和AgNORs的观察

The Observation of Proliferative Cell Nuclear Antigen and AgNORs in Ocular Adnexal Lymphoid Hyperplasia

目的:探讨眼部淋巴组织增生性病变的增殖活性与组织学分型的关系。方法:应用抗增殖细胞核抗原的单克隆抗体PC1O和AgNORs技术,对34例眼附属器淋巴组织增生性病变(包括良性淋巴组织增生14例,不典型增生9例,恶性淋巴瘤11例)的石腊切片进行检测。结果:良性淋巴组织增生与后二者PCNA及AgNORs计数之间均有非常显著的差异(PCNA P<0.001,AgNORs P<0.01及P<0.001),不典型增生与恶性淋巴瘤之间PCNA计数相差非常显著(P<0.01),AgNORs颗粒数量则无显著差异(P>0.05),同时发现AgNORs与PCNA计数有良好的相关关系(0.8693)。结论:两种技术对眼部淋巴增生病组织学分型有一定参考价值。眼科学报1998;14:66—68。

Purpose: To investigate the expression of proliferative cell nuclear antigen and Nuclear Organizer Region in ocular adnexal lymphoid hyperplasia and its histopathologic classification. Method: Using anti - proliferative cell nuclear antigen monoclonal antibody ( PC10 ) and AgNORs method, the proliferative cell in 34 cases of ocular adnexal lymphoid neoplasms (benign lymphoid lesion, BLL, 14 cases; atypical lymphoid hyperplasias, AH, 9 cases; malignant lymphomas, ML, 11 cases) wrer detected in formalin fixed and paraffin- embeded tissuse. Results:A significant difference between BLL and AH, BLL and ML was observed (PCNAP<0.001, P<0.001; AgNORs P<0.01, P<0.001); similar result was observed between AH and ML in PCNA index (P<0. 01). But there was not a significant difference in AgNORs count (P>0.05). The linear regression coefficient between PCNA index and AgNORs number was excellent (0.8693). Conclusion :The two methods may be helpful to distinguish ocular adnexal lymphoid reactive hyperplasia and malignant lymphoma. Eye Science 1998 ; 14 : 66 - 68.