人眼睫状肌细胞培养及生物学特性的研究

Study on Human Eye Ciliary Muscule Cell Culture and Biologic Characteristics

目的:培养人眼睫状肌细胞,研究其生长、超微结构、免疫组织化学特性和功能特点。方法:采用组织块培养法对10只青年供体眼睫状肌进行培养,观察其生长特性,用透射电子显微镜检查其超微结构,进行结蛋白免疫组织化学检查,应用单细胞收缩分析技术研究卡巴可对细胞收缩的影响。结果:从人眼睫状肌组织培养了细胞并传代,细胞融合后呈峰谷样排列;透射电子显微镜下可见丰富的微丝;培养的细胞结蛋白染色阳性,组织切片中睫状肌细胞结蛋白染色阳性,成纤维细胞,血管内皮细胞结蛋白染色阴性,小血管壁呈弱到中度阳性。10^(-3)M卡巴可可以引起培养的细胞收缩,此作用被10^(-3)M阿托品拮抗。结论:培养的细胞符合人眼睫状肌细胞特征,具有收缩功能。眼科学报1998;14:69—72。

Purpose: We cultured human ciliary muscle [HCM] cells to study their growth, ultrastructure, immunohistochemistry and functional characters. Methods: HCM cells from 10 young donor eyes were cultured with collagenase IV digestion procedures in vitro, the cells were identified by eletronmicroscope and immunohistochemistry assay, their function were studied by single - cell - contraction assay. Results:The cells were passed and grew in Hill - Valley pattern after conflunet; abundant filaments were presented under electronmicroscope. In Desmin protein immunohistochemistry study, the cultured cells were stained positive; in tissue sections, HCM cells stained positive, vascular smooth muscle stained positive weakly, but fibroblast cells and endothelial cells stained negative. 10-3M Carbachol could induce the cultured cells contract, this effect was antagonized by 10-3 M Atropine. Conclusion:We successfully cultured HCM cells, which were able to contract. Eye Science 1998; 14:69-72.