人眼小梁细胞体外培养、冻存与复苏

The Study on the Culture, Freezing Preservation and Resuscitation of Human Trabecular Cells in Vitro

目的:建立人小梁细胞体外培养及对其进行冻存、复苏研究。方法:应用组织块培养方法进行人小梁细胞体外培养,将第3代的小梁细胞冻存,冻存1周、2周、1月、2月后细胞给予复苏,观察复苏后细胞的生长情况。结果:人眼小梁细胞体外培养成功。冻存的小梁细胞复苏成功,所有复苏率超过90％。结论:人眼小梁细胞体外培养及冻存复苏成功,为以后构建小梁细胞的cDNA文库,为筛选青光眼发病的相关基因提供有利的实验基础。眼科学报1998;14:73—75。

Purpose: To establish the culture of human trabecular cells in vitro and study the freezing preservation and resuscitation. Methods: The trabecular specimens from human eyes were cultured in vitro and passaged 3 times, then the cultured cells were frozen and preserved. The frozen cells were resuscitated after one week, two weeks, one month and two months. Results: Cultured human trabecular cells were obtained. The frozen cells were resuscitated successfully. All of the resuscitated rates were more than 90%. Conclusion:The human trabecular cells were cultured in vitro successfully, they can also be frozen and resuscitated, which will be helpful to the establishment of cDNA library and the screening of associated glaucomatous genes. Eye Science 1998; 14 : 73 - 75.