金银花炮制机理的初步探讨

Preliminary study on the mechanism of processing Flos Lonicerae Japonicae

目的:研究炮制对金银花化学成分的影响,为更深入研究该药炮制机理提供实验依据。方法:制备金银花炒黄品、烘制品,采用HPLC-DAD方法以绿原酸为指标性成分,通过指纹图谱对比对生品和炮制品成分进行分析比较。结果:金银花生品炒黄后,大部分成分溶出率增加;而金银花生品经烘制品后,大部分化学成分含量降低,少数化合物含量升高。结论:炮制对金银花的化学成分影响较大,可为找到抗菌活性最强的成分提供实验线索。

Objective： To Study on the effect of processing on the chemical constituents of Flos Lonicerae japonicae in order to provide a proof for research on the mechanism of processing Flos Lonicerae Japonicae.Methods： The processed products of Flos Lonicerae japonicae were prepared,and the fingerprint chromatograms of crude and processed Flos Lonicerae Japonicae were compared by HPLC-DAD.Results： Compared with crude drugs,the compositions in stir-heating drugs had the higher dissolution rate,and the most of compositions in baking drugs had lower dissolution rate,even disappeared,and some of compositions had obviously higher dissolution rate.Conclusion： Processing has great influence on the chemical constituents of Flos Lonicerae japonicae,and it provides a key for figuring out chemical compositions with better antibacterial activity in baking drugs.