G蛋白偶联受体30和磷酸化AKT在子宫内膜腺癌中表达及意义

The expressions of G protein-coupled receptor 30 and phosphorylated AKT in endometrial adenocarcinoma

目的:探讨G蛋白偶联受体30(GPR30)和磷酸化AKT(P-AKT)在子宫内膜腺癌发生发展中的作用及相互关系。方法:用免疫组化SP法检测10例增生期子宫内膜,49例子宫内膜增殖症,55例子宫内膜腺癌组织中GPR30和P-AKT的表达。结果:GPR30蛋白在子宫内膜腺癌、子宫内膜增殖症的阳性表达率(81.8%、67.3%)均明显高于增生期子宫内膜中的阳性表达率(20.0%);子宫内膜增殖症中GPR30蛋白在单纯型增生子宫内膜(SH)、复杂型增生子宫内膜(CH)和不典型增生子宫内膜(AH)的阳性表达率分别为30.0%(3/10),70.0%(14/20)和84.2%(16/19),AH和SH的差异有统计学意义(P=0.012)。P-AKT在子宫内膜腺癌、子宫内膜增殖症的阳性表达率(78.2%、71.4%)均显著高于增生期子宫内膜中的阳性表达率(20.0%);子宫内膜增殖症中P-AKT蛋白在SH、CH、AH的阳性表达率分别为40.0%(4/10),65.0%(13/20)和94.7%(18/19),AH与SH的差异有统计学意义(P=0.005)。子宫内膜腺癌GPR30、P-AKT蛋白阳性表达率与组织分化程度、FIGO分期及患者是否绝经有关,在中、低分化组(P=0.023;P=0.009)、FIGOⅡ～Ⅲ期(P=0.039;P=0.017)及绝经后(P=0.046;P=0.031)较高。肌层浸润较深的子宫内膜腺癌P-AKT蛋白表达水平高于肌层浸润较浅者(P=0.042)。子宫内膜腺癌组织中GPR30与P-AKT蛋白表达呈正相关(P<0.001)。结论:GPR30和P-AKT活化与子宫内膜癌的发生发展有关。

Objective：To investigate the expressions of G protein-coupled receptor 30（GPR30）and phosphorylated AKT（P-AKT）in endometrial adenocarcinoma and to investigate their effects on endometrial tumorigenesis and development.Methods：Expressions of GPR30 and P-AKT protein were detected by immunohistochemistry in 10 cases of proliferative phase endometrium,49 cases of endometrial hyperplasia and 55 cases of endometrial adenocarcinoma.Results：The positive rates of GPR30 in endometrial adenocarcinoma（81.8%）and endometrial hyperplasia（67.3%）were significantly higher than that of the proliferative endometrium（20.0%）respectively（P0.001;P=0.015）.As to endometrial hyperplasia,positive staining of GPR30 in SH,CH and AH was 30.0%（3/10）,70.0%（14/20）and 84.2%（16/19）,respectively.The positive rate of GPR30 in AH was significantly higher than that in SH（P=0.012）.The expressions of P-AKT in endometrial adenocarcinoma（78.2%）and hyperplasia（71.4%）were significantly higher than that of the proliferative endometrium（20.0%）respectively（P=0.001;P=0.007）.In SH,CH and AH,the positive immunostaining of P-AKT was 40.0%（4/10）,65.0%（13/20）and 94.7%（18/19）respectively.The positive rate of P-AKT in AH was significantly higher than that in SH（P=0.005）.The positive rate of GPR30 or P-AKT in poor histological differentiation（P=0.023;P=0.009）,late clinical stages（P=0.039;P=0.017）and postmenopause group（P=0.046;P=0.031）was higher than in well differentiation,early clinical stages and premenopause group.In addition,the positive rate of P-AKT was higher in endometrial adenocarcinoma with deep myometrial invasion（P=0.042）.The expression of GPR30 was positively correlated with P-AKT expression in endometrial adenocarcinoma（P0.001）.Conclusion：The activation of GPR30 and P-AKT may be correlated with the genesis and development of endometrial adenocarcinoma.