B7-H4和FOXP3在乳腺癌中的表达及临床意义

Expression and clinical significance of B7-H4 and FOXP3 in breast cancer

目的:研究B7-H4和FOXP3在乳腺癌中的表达及其相互关系和临床意义。方法:应用S-P免疫组化法检测B7-H4蛋白和FOXP3蛋白在11例正常乳腺、25例乳腺良性病变、272例乳腺癌组织中的表达情况;运用原位杂交检查B7-H4mRNA在10例正常乳腺、10例良性病变、20例乳腺癌组织中的表达情况。结果:B7-H4蛋白在乳腺癌中的阳性表达率(83.39%)高于乳腺良性病变组(64.00%)和乳腺正常组(54.55%),差异有统计学意义(p<0.05);FOXP3在乳腺癌中的阳性表达率(82.67%)高于乳腺良性病变组(60.00%)和乳腺正常组(54.55%),差异有统计学意义(p<0.05)。;B7-H4和FOXP3在乳腺癌中的阳性表达率与患者年龄、肿块大小和组织学分型无关(p>0.05),而与组织学分级和淋巴结转移相关(p<0.05);B7-H4和FOXP3在乳腺癌组织中的表达呈正相关(r=0.306,p<0.001);B7-H4mRNA在正常乳腺组、乳腺良性病变组、乳腺癌组中的阳性表达率差异没有统计学意义(p>0.05);B7-H4mRNA在乳腺癌中的阳性表达率与年龄、肿块大小、组织学分级、淋巴结转移无关(p>0.05)。结论:B7-H4和FOXP3在乳腺癌中表达上调,可能与乳腺癌的转移和预后相关。

Objective：To study the expression of B7-H4 and FOXP3 in breast carcinoma ,and texplore their relationship and clinical significance. Methods： The expression of B7-H4 protein and FOXP3 protein was evaluated by immunohistochemistry in 11 cases of normal breast, 25 cases of benign lesions, and272 cases of breast carcinoma; The expression of B7-H4 mRNA was measured by in situ hybridization in 10 cases of normal breast, 10cases of benign lesions and 20 cases of invasive breast cancer. Results： The positive rate of B7-H4 protein and FOXP3 protein in breast cancer was significantly higher than that in benign lesions and normal breast （p〈0.05）; There was no correlation between the positive rate of B7-H4 protein and FOXP3 protein in breast cancer and age, tumour size, histological classification （p〉0.05）, but the expression of B7-H4 protein and FOXP3 protein was positively correlated to histological grade and lymph node matastasis （p〈0.05）; The expression of B7-H4 in breast cancer was significantly positive correlated with FOXP3（r=0.306,p〈0. 001）; There was no statistical significance on positive rate of the expression of B7-H4 mRNA between breast cancer, benign lesions and normal breast （p〉0.05）; There was no statistical significance between the positive rate of B7-H4 mRNA and age, tumour size, histological classification, histological grade and lymph node matastasis （p〉0.05）. Conclusions： The expression of B7-H4 protein and FOXP3 protein in breast cancer is higher than that in normal breast and in benign lesions. The results suggeste that B7-H4 and FOXP3 may be closely related to metastasis and prognosis.