摘要
目的:探讨低聚原花青素(OPC)对1型糖尿病(T1DM)小鼠血脂代谢和胰岛功能的影响及意义。方法:用链脲佐菌素(STZ)小量多次腹腔注射制作T1DM小鼠模型;实验组分3个剂量每天灌胃OPC液,模型对照组和正常对照组则灌胃等容积蒸馏水。4周末酶法检测动物血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)含量;免疫组织化学法检查胰腺组织胰岛中胰岛素蛋白的表达。结果:与模型对照组比较,OPC给药组症状改善;血清TC、TG和LDL-C明显降低(P<0.05,P<0.01);HDL-C有所增加,但不具统计学差异(P>0.05)。胰腺组织免疫组化染色结果显示:模型组胰岛中有多量淋巴细胞浸润,仅少量残留的胰岛细胞被染成棕色,胰岛素表达明显减少;OPC组胰岛中着色的胰岛细胞明显增多,胰岛素表达明显增加,淋巴细胞浸润明显减少。结论:OPC能够降低糖尿病小鼠的血脂含量,提高胰岛分泌功能,减轻由STZ诱导的自身免疫反应,有利于改善糖尿病症状和减少糖尿病心血管并发症的发生。
Objective:To study the effects of OPC on blood lipid and Islet function in Type 1 diabetes(T1DM) mice.Methods:T1DM was induced in male KM mice with multiple low dose streptozotocin(ld-STZ)by i.p.administration;to treat the T1DM mice with oligomeric proanthocyanidin(OPC)in doses of 50、100and 150mg/kg respectively by oral administration once every day for 4 weeks.Then the content of TC、TG、LDL-C、HDL-C in serum of experiment mice were measured;The expressions of Insulin on islet cells were detected by immunohistochemical Straining.Results:The effect of OPC in doses of 100 and 150mg/kg on reducing serum lipid content in test group was statistically different(P <0.05,P<0.01, espectively )in comparison with DM control group,while the expressions of Insulin on islet cells were obviously stronger than that in test group.Conclusion:OPC has the effects of decreasing the concentration of blood lipid and repairing the injury of islet cells of T1DM mice.
基金
湖北省科技厅计划项目(2006AA402CO2)
关键词
低聚原花青素
1型糖尿病
血脂
胰岛素
Oligomeric proanthocyanidin
Type 1 diabetes mellitus
Blood lipid
Insulin
