摘要
目的:观察局灶性脑缺血再灌注后Nogo-A mRNA和蛋白表达的动态变化,以及三七三醇皂苷(PTS)对其影响。方法:将健康雄性SD大鼠随机分为假手术组、模型组和药物干预组(PTS组),采用Longa线栓法制备大脑中动脉阻塞与再灌注大鼠短暂局灶性脑缺血模型。药物干预后,采用RT-PCR结合免疫组织化学技术检测大鼠缺血再灌注后不同时期(3 d,7 d)脑组织Nogo-A mRNA和蛋白的表达。结果:模型组Nogo-A mRNA和蛋白表达在缺血再灌注损伤后3 d时下降,7 d时上升。PTS组在3 d时大脑皮层和海马Nogo-A表达比模型组低,但差异无显著性意义(P>0.05);7 d时Nogo-A表达明显低于模型组,差异有显著性意义(P<0.01)。结论:PTS可使缺血再灌注大鼠的Nogo-A表达下降,这可能是其发挥脑保护作用的机制之一。
Objective ：To investigate the dynamic changes in Nogo-A mRNA and protein expression in brain tissue of rats with focal cerebral ischemia-reperfusion as well as the effects of PTS （PTS） on it. Methods：The male SD rats were randomly divided into sham operation group, model group and drug intervention group （PTS group）, and the model of acute reperfusion injury after cerebral ischemia in rats was established by middle cerebral artery occlusion. After drug intervention, the mRNA and protein expression of Nogo-A in the brain tissue was determined by RT-PCR and immunohistochemistry in different periods of reperfusion （3 d, 7 d）. Results： The mRNA and protein expression of Nogo-A in model group was decreased on the third day and increased significantly on the seventh day ; while, in the PTS group, the expression of Nogo-A was decreased on both the third day and seventh day, with the former similar to that of the model group （P 〉 0.05 ） and the latter significantly lower than that of model group（ P 〈 0.05）. Conclusion：The PTS can down-regulate the expression of Nogo-A in rats with ischemia-reperfusion injury, which may be one of the mechanisms for its protection to brain.
出处
《上海中医药大学学报》
CAS
2010年第1期59-62,共4页
Academic Journal of Shanghai University of Traditional Chinese Medicine
基金
上海中医药大学附属普陀医院科研基金资助项目(2007J026A)
