UV-C辐照抑制铜绿微囊藻生长的动态实验研究

Dynamic Experiment on Controlling of Microcystis aeruginosa by UV-C Irradiation

以我国典型淡水水华藻种铜绿微囊藻（Microcystis aeruginosa 905）为研究对象，采用推流式低压紫外灯反应器研究在动态条件下Ultraviolet—C（UV—C）辐照对蓝藻生长抑制作用的效果．对数培养期的蓝藻液流经反应器进行UV—C辐照处理，其后正常培养，在培养期2h、1d、3d、5d、7d、9d分别取样，通过研究级倒置荧光显微镜在荧光视野下进行细胞计数．结果表明，辐照处理未引起铜绿微囊藻细胞的大量破裂死亡，36～115mw·s·cal2剂量范围的UV—C辐照能在9d内有效抑制2．6×10^5～2．7×10^5cells·mL^-1的铜绿微囊藻细胞密度增长，31～50mw·s·cm^-2剂量范围的UV—C辐照能在9d内有效抑制9．0×10^5～1．15×10^6cells·mL^-1的铜绿微囊藻细胞密度增长．

A plug-flow UV-C reactor equipped with low pressure UV lamp was utilized to study the suppression effect after UV-C irradiation under the dynamic conditions on Microcystis aeruginosa, a typical cyanobacterium in algae blooms in China. The culture fluid of Microcystis aeruginosa was exposed to UV-C irradiation when pumped through the reactor. After that, the fluid was incubated under the normal culture condition, and sampled at 2 h, 1 d, 3 d, 5 d, 7 d, 9 d for determination of cell density using the inverted system fluorescence microscope. The experiments showed that, UV-C irradiation did not cause severe cell lysis, and UV-C irradiation at dose ranged from 36 to 115 mW · s · cm^-2 , and 31 to 50 mW · s · cm^-2 could suppress Microcystis aeruginosa biomass growth for the 2.6 × 10^5-2.7 × 10^5cells · mL^-1 and 9.0 × 10^5-1. 15 × 10^6cells · mL^-1 fluid in 9 days, respectively.