胎盘免疫调节因子对HepG 2.2.15细胞毒性及HBV DNA分泌作用的影响

Effect of placenta immunoregulating factor on the cytotoxicity and HBV DNA secretion in the HepG2.2.15 cells

目的观察胎盘免疫调节因子（PF）在体外的抗乙肝病毒（HBV）作用及安全性。方法将质量浓度分别为0．032—20．000mg／ml的PF作用于体外培养的人肝癌细胞系HepG 2．2．15细胞，通过MTT比色法观察细胞增殖抑制率及半数毒性质量浓度（TC50），以荧光定量PCR法检测HBV DNA水平（以拉米夫定为阳性对照组）。结果PF处理后细胞增殖抑制率为0．75％～58．21％且呈浓度依赖性，作用72h后TC50为13．61mg／ml；PF作用72h和144h后，细胞上清液中HBV DNA拷贝量显著降低，与阳性对照组水平相似。结论PF在体外有显著抗HBV作用，且细胞毒性较小。

Objective To observe the antihepatitis B virus （HBV） effects of placenta immunoregulating factor（PF） in vitro. Methods The human hepatoma cell line HepG 2.2.15 cells cultured in vitro were exposed to PF at the mass concentrations of 0. 032 to 20.000 mg/ml respectively. The inhibition rate and half of the inhibition rate of toxic concentration （TC50） of cells were evaluated by Methyl thiazolyl tetrazolium（MTT） assay. The HBV DNA level was detected by Fluorescence quantitative-PCR（ with Lamivudine. as a positive control group）. Results The cell inhibition rate was 0.75% to 58.21% in concentration-dependent manner after treated by PF,TCs0 72 hours after the treatment was 13.61 mg/ml. Seventy-two and 144 hours after treated by PF,the copies of HBV DNA in the supematant of the cells decreased significantly,and the level was similar to the positive control group. Conclusion PF has significant anti-HBV effect in vitro, and appeares less cytotoxicity.