青蒿琥酯对人胚肺成纤维细胞系HFL-I细胞增殖、凋亡的影响及机制

Effects and mechanism of artesunate on the cell proliferation and apoptosis in human embryonic lung fibroblast cell line HFL-I

目的进一步探讨青蒿琥酯的抗纤维化作用及机制。方法取对数生长期人胚肺成纤维细胞（HELF）系HFL-I细胞株，随机分为观察1—4组及对照组，观察1-4组分别加入质量浓度为4、8、16、32mg／L的青蒿琥酯，对照组加入等体积培养液后继续培养。用CCK-8法检测细胞增殖情况（A值），流式细胞仪检测细胞周期及凋亡率，RT-PCR法测定Survivin mRNA表达。结果观察1-4组G0＋G1期细胞所占比例及细胞凋亡率均显著高于对照组，细胞A值及Survivin mRNA表达均显著低于对照组，且各观察组间亦有显著差异（P〈0．05）。结论青蒿琥酯具有抗肺纤维化作用，可能机制为通过下调Survivin mRNA表达抑制HFL-I细胞增殖、促进HFL-I细胞凋亡。

Objective To investigate the antifibrotic action and mechanism of artesunate furtherly. Methods Human embryonic lung fibroblasts （HELF） ceil lines HFL-I were cultured to log phase in vitro and divided into 4 observed groups and control group , the former 4 groups were treated with artesunate at the mass concentration of 4,8,16,32 mg/L, the control group were given equivalent DMEM. The cell proliferation activity was determined by CCK-g assay, the cell cycle and apoptosis ratio were detected by flow eytometry（FCM） ,the expression of Survivin mRNA was detected by RT-PCR. Results Compared with the control group, percentage of G0 ＋ G1 stage cells and the apoptosis ratio in observed groups increased significantly,A value and the expression of Survivin mRNA decreased significantly, and remarkable differences were also seen among observed groups （ P 〈 0.05 ）. Conclusion Artesunate can resist pulmonary fibrosis, and probable mechanism relate with proliferation inhibition and apoptosis induction of HFL-I through downregulating expression of Survivin mRNA.