简化RP-HPLC法同时测定茶碱和可可碱的含量

Improved RP-HPLC Method for the Determination of THO and TB Concentrations

确定了同时测定茶碱和可可碱含量的HPLC条件：Symmetry C18柱，乙腈-水（10：90）为流动相，检测波长275nm，流速1mL／min。在此条件下茶碱（THO）的线性回归方程为y=3．186×10^6x-1．025×10^5，r=0．9999）；可可碱（TB）回归方程：y=3．011×10^6x-4．092×10^4（r=0．9998），进样量分别在0．09～1．35μg和0．036-0．36μg范围内与峰面积值呈良好线性关系。

The optimum HPLC conditions for determining the concentrations of THO and TB in methanol solution were obtained with Symmetry C18 as column＇ acetonitrile-water （1 ： 9） as fluid phase, 275 nm as determining wavelength and 1 mL/min as flow rate. The linear regression equation was y=3. 186 × 10^6x- 1. 025 ×10^5 （r=0. 9999, injection amount 0. 09- 1.35 μg） for THO and y= 3.011 × 10^6x-4. 092 ×10^4 （r= 0. 9998, injection amount 0. 036-0.36 μg） for TB.