鼠颅面部矿化组织发育中非胶原蛋白的表达

EXPRESSION OF NON-COLLAGENOUS PROTEINS DURING DEVELOPMENT OF CRANIOFACIAL MINERALIZED TISSUES OF RATS

目的：骨涎蛋白和骨桥蛋白是矿化组织基质中的主要非胶原蛋白，本研究旨在对它们在矿化组织发育和形成中的可能作用作一探讨。方法：采用免疫组织化学和分子原位杂交技术对各发育阶段的鼠颅面部矿化组织中的骨涎蛋白和骨桥蛋白进行检测。结果：骨涎蛋白主要在新生骨的成骨细胞和类骨质以及前期牙本质中表达；而骨桥蛋白则可在骨形成和骨改建部位的成骨细胞和类骨质以及前期牙本质中出现。结论：结果表明骨涎蛋白与成骨和羟基磷灰石的形成有关；而骨桥蛋白则在矿化形成的不同阶段发挥多重作用。

Bone sialoprotein (BSP) andOsteopontin (OPN) are two major non-collagenous proteins of mineralized tissues matrix. The goal of the presentstudy was to investigate the possible effects of LSP andOPN in the development and formation of mineralized tissues. METHODS: The location of BSP and OPN on craniofacial mineralized tissues of rats in various developmentalperiods were detected used immunohistochernistry and insitu hybridization techniques. RESULTS: BSP signals wereshown in the osteoblasts and osteoid matrix of newly-forming bone as well as in the predentine. OPN, however, appeared in the osteoblasts and osteoid, both bone formationand remodeling, and predentine. CONCLUSION: The results suggest that BSP participates in the bone formationand hydroxyapatite growth, and OPN plays a variety ofroles in the different stages of mineralized tissues development.