HPLC法测定脂质体松萝酸中松萝酸的含量

HPLC determination of usnic acid in liposomal usnic acid formulations

建立了HPLC测定脂质体松萝酸中药物松萝酸的方法。以KromasilC18反相色谱柱（250mm×4．6mm，5μm）为分离柱，流动相组成为V（甲醇）：V（磷酸盐缓冲液（pH5．0））=7：3，流速1mL／min，紫外检测，波长284n／n。松萝酸在10-50倒mL的范围内有很好的线性关系（r=0．9994）。加样回收率为100．1％±0．6％，RSD为0．54％4±0．07％。本方法适用于脂质体松萝酸中药物松萝酸的测定。

To establish a HPLC method for the detection of usnic acid in liposomal usnic acid. Kromasil C18 column （250 mm×4.6 mm, 5 μm） was used, and the mobile phase consisted of MeOH-PBS （pH 5.0） was 7：3 （V/V）. The flow rate was 1.0 mL/min and the determination wavelength was at 284 nm. The linear range was in the range of 10 - 50μg/mL （ r = 0. 9994）. The average recovery was 100.1% ± 0.6% ; RSD was 0.54% ±0.07%. This method is simple, rapid and reliable for the determination of usnic acid in liposomal usnic acid formulations.