摘要
目的探讨温肾清卫颗粒对特发性血小板减少性紫癜(ITP)患者血小板生成素(thrombopoietin,TPO)、血小板相关抗体IgG(platelet-associated immunoglobulin G,PAIgG)及TPO受体c-MPL mRNA水平的影响。方法将60例患者随机分为治疗组(温肾清卫颗粒)、对照组(强的松)各30例,同时以20例健康志愿者为正常组。两治疗组经治1个月、3个月、6个月后,分别采用ELISA法检测血清TPO、PAIgG水平,采用半定量RT-PCR法检测外周血血小板c-MPL mRNA的相对量。结果治疗1个月时,治疗组血小板计数低于对照组(P<0.05);治疗3个月及6个月时,治疗组血小板计数高于对照组(P<0.05)。治疗1个月时,治疗组血清TPO、PAIgG水平高于对照组(P<0.05,P<0.01),两组血小板c-MPL mRNA水平比较无明显差异(P>0.05);治疗3个月、6个月时,治疗组血清TPO、PAIgG水平均低于对照组(P<0.05,P<0.01),血小板c-MPL mRNA均高于对照组(P<0.05,P<0.01)。结论温肾清卫颗粒可能通过抑制ITP患者PAIgG表达以及调控c-MPL mRNA基因转录,促进血小板生成增加。
Objective To investigate the effect of ＂Wenshen Qingwei Granule＂ on serum PAIgG, TPO and its receptor c-MPL mRNA changes in patients with ITP. Methotis A total of 60 patients were randomly divided into two groups： treatment group in which 30 cases were treated with ＂Wenshen Qingwei Granule＂ and control group in which 30 cases were treated with prednisone; and 20 healthy people were enrolled as normal group. The serum TPO and PAIgG in the two groups were detec- ted by ELISA respectively after one-month, three-month and six-month treatments. The c-MPL mRNA in platelets was determined by semi-quantitative reverse transcription polymerase chain reaction（RT-PCR） in the two groups. Results After one-month treatment, the expressions of TPO and PAIgG in serum were higher and platelets quantity was lower in the treatment group than those in the control group（P 〈0.05 or P 〈0.01 ） ; c-MPL mRNA in platelets was of insignificant difference between two groups（ P 〉0.05 ）. After three-month and six-month treatments, the TPO and PAIgG in serum were decreased and c-MPL mRNA levels in platelets were increased in the treatment group compared with those in the control group（P 〈0.05 or P 〈0.01 ）, while the platelets quantity was elevated （P 〈0.05 or P 〈0.01 ）. Conclusion ＂Wenshen Qingwei Granule＂ can inhibit PAIgG exoression and c-MPL mRNA transcriotion to increase the platelet production.
出处
《上海中医药杂志》
2010年第1期53-55,共3页
Shanghai Journal of Traditional Chinese Medicine
