植物病原菌拮抗放线菌的分离筛选与鉴定

Isolation, Screening and Identification of Antagonistic Actinomycetes Inhibiting Plant Pathogens

从杭州地区采集的11份土样中共分离到99株放线菌,筛选出9株对产核黄素的无名假丝酵母Candida famata有拮抗作用。采用平板对峙法复筛到1株对供试的多种植物病原菌抑制效果较强的菌株W04,该菌株对西瓜枯萎病原菌的抑菌率达到79.1%;生长速率法测得其发酵滤液对西瓜枯萎病原菌的抑制率为82.6%。根据形态特征、生理生化特性及16SrDNA序列分析,鉴定该菌株为淡紫灰链霉菌Streptomyces lavendulae Waksman & Curtis。

A total of 99 actinomycetes were isolated from 11 kinds of soil samples collected from Hangzhou areas. Nine actinomycetes were screened, which had antagonism to Candida famata producing riboflavin. The strain W04 which had better inhibitory effects to many pathogenic pathogens tested was re-screened by panel confrontation method. The inhibition rate against Fusarium oxysporum f. sp. niveum reached 79.1%, and the mycelia growth inhibitory rate of its fermention filtrate was 82.6% using growth rate method. The strain W04 was identified as Streptomyces lavendulae Waksman ＆ Curtis according to morphology, physiology biochemistry and 16S rDNA sequence analysis.