摘要
为了建立ＲＡＰＤ技术分析利什曼原虫ＤＮＡ多态性的方法，并进一步揭示Ｌｉｎｆａｎｔｕｍ和Ｌｔｒｏｐｉｃａ之间的亲缘关系，本文应用筛选的两种随机引物（Ｍ１３，３３０１）分别扩增了Ｌｉｎｆａｎｔｕｍ和Ｌｔｒｏｐｉｃａ的ｎＤＮＡ和ｋＤＮＡ。结果显示，两种利什曼原虫ｎＤＮＡ及ｋＤＮＡ均扩增出各自特有的带型，两虫种间ｎＤＮＡ、ｋＤＮＡ的扩增片段共享度Ｆ值分别为０７２７２和０８５７１。
The random amplified polymorphic DNA (RAPD) assay has been used to find molecular markers and to detect L.infantum and L.tropica. Both nuclear DNA and kinetoplast DNA were analysed by RAPD using two arbitrary primers.The results showed that the two primers yielded characteristic patterns for nDNA and kDNA between L.infantum and L.tropica and there were some differences of the share random amplified DNA fragments, which demonstrated that RAPD PCR was a useful tool to analyse Leishmania DNA.
出处
《实用寄生虫病杂志》
1998年第3期100-102,共3页
Journal of Practical Parasitic Diseases
基金
国家自然科学基金
四川省卫干院科研基金
