摘要
目的：将Ｆ１ＳＨ方法应用于精子间期核检测染色体数目。方法：用二硫苏糖醇打破精子表面蛋白壳，使探针容易穿透，再选用１３、２１、ｘ探针与精子核杂交，检测相应染色体数目。结果：可见红色核中绿色信号，结果直观，能准确计数染色体数目。结论：ＦＩＳＨ技术应用于精子间期核，能准确、快速、直观地检出染色体数目异常。
Objective: Using fluorescence in situ hybridization (FISH) detects numerical chromosome abnormalities in human sperm. Methods: Choosing chromosome specific for 21/13 and X DNA probes hybridized with normal human sperm. Results: We can see the yellow signals in the red neuclei. It can be calculated easily. Conclusion: Using FISH may accurately detects numerical chromosome abnormalities in human sperm by counting the number of fluorescent spots.
出处
《内蒙古医学院学报》
1998年第3期153-155,共3页
Acta Academiae Medicinae Neimongol
关键词
精子
荧光原位杂交
染色体
人
fluorescence in situ hybridization
spermatozoa
chromosomes
human
