摘要
目的探讨献血员中一种与输血后肝炎有关的病毒—TTV的感染情况。方法采用TTV基因组ORF1区的套式聚合酶链反应(nested-PCR)方法检测262份献血员血清标本。结果在血清丙氨酸转氨酶(ALT)异常、HBsAg及抗-HCV阴性的58份献血员血清标本中,18份TTVDNA阳性,TTVDNA的阳性检出率为310%;在204份正常献血员血清标本中检出30份(147%)TTVDNA阳性,TTVDNA的阳性检出率明显低于ALT异常献血员人群(χ2=381,P<005)。序列分析结果显示,其序列与日本株和中国株相应位置核苷酸序列的同源性大于97%,可能属同一基因型。结论提示我国献血员中存在TTV感染,TTV存在“健康携带状态”。
In order to investigate TTV infection in blood donors, 262 sera samples from blood donors were detected by nested-PCR method using primers from ORF1 of TTV genome DNA. The results showed that 18 of 58(31 0%) serun samples from blood donors with elevated transaminase level were positive for TTV DNA. 30 of 204 (14 7%) sera samples from blood donors with normal transaminase levels were positive for TTV DNA. There were significant difference between them (χ 2=38 1,P<0 05). Five products of PCR for TTV DNA were purified and cloned to T Vector. The sequence analysis showed that there were above 97% nucleotide identity among AB008394 (from Japan),TTVCHNI(from China) and our 5 isolates from blood donors. The results suggest that there exists TTV infection among blood donors in China. TTV may be transmitted by blood transfusion. Our isolates, AB008394 and TTVCHN1 share a same genotype.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
1998年第3期237-240,共4页
Chinese Journal of Experimental and Clinical Virology
关键词
TT病毒
献血员
聚合酶链反应
核苷酸序列
Transfusion-transmitted virus Blood donors Polymerase chain reaction Nucleotide sequence