摘要
目的研究庚型肝炎病毒(HGV)在恒河猴中的实验感染状态。方法用一名HGVRNA阳性、HBV、HCV均阴性的健康献血员血浆实验感染2只恒河猴,并取第一代猴感染后6周的血再感染1只第二代恒河猴,然后用以第二代猴感染6周后血继续感染2只第三代恒河猴。分别用逆转录聚合酶链反应(RT-nPCR)检测受感染猴血清中的HGVRNA,并每周抽血测定血清中丙氨酸转氨酶(ALT)。结果感染1周后猴血清HGVRNA阳转,最长持续阳性28周以上。不同感染个体血清ALT水平有明显差异,其中1号猴有短期轻度升高,5号猴血清ALT较长时间在100U/L以上。肝活检发现,感染后16周猴肝组织出现明显的病毒性肝炎样病理改变。进一步对该献血员血浆和感染后猴血清中的HGV5’端部分非编码区基因PCR产物进行测序,结果显示感染用献血员血浆和猴血清中HGV序列与国外株HGU44402的同源性分别为9833%和9583%;与HGU36380株的同源性分别为9250%和8917%;感染猴血清中HGV序列与献血员HGV序列同源性为9583%。结论恒河猴对HGV敏感。
In order to study the experimental infection of HGV in rhesus monkey, the monkeys were infected using the plasma from a donor with HGV RNA positive. The second generation monkeys were infected with the sera of the first generation monkeys infected after 6 weeks, and also the third generation monkeys were infected with the sera of the second generation monkeys. HGV RNA were detected by RT-nPCR. The results showed that in sera of infected monkeys HGV RNA were positive after 1 week of inoculation, and were consecutively positive for up to 28 weeks at longest. The level of ALT raised a little in monkey No.1 and higher than 100U/L in No.5. The liver biopsy showed viral hepatitis-like histological changes. Comparing the sequence of HGV 5'LTR from sera of the infected monkeys and the blood donor, the homogeneity, to strain HGU44402 was 98 33% and 95 83% to strain HGU36380 was 92 50% and 89 17%, respectively. The results suggested that the rhesus monkey is sensitive to HGV and is suitable for establishment of an animal model.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
1998年第3期258-260,共3页
Chinese Journal of Experimental and Clinical Virology
关键词
庚型肝炎病毒
恒河猴
实验感染
Hepatitis G virus Rhesus monkey Experimental infection