耐三苯氧胺大鼠乳腺癌细胞株的生物学特性

The Biological Characterization of a Tamoxifen-Resistant Rat Breast Cancer Cell Strain

目的：建立耐ＴＡＭ的大鼠乳腺癌细胞株，有助于阐明其耐药性机制。方法：应用改良的Ｈｕｇ双层琼脂方法，对ＳＨＺ－８８细胞进行克隆培养，筛选出耐受ＴＡＭ的大鼠乳腺癌细胞。结果：该细胞株对ＴＡＭ的敏感性比原代细胞降低１０倍，但对Ｅ２的反应性无改变。结论：该细胞株的建立有助于研究耐药乳腺癌细胞的生物学行为。

Objective:The establishment of the tamoxifen-resistant rat breast cancer cell strain will be helpful to elucidate the mechanism of drug resistance.Method:A tamoxifen-resistant rat breast cancer cell strain was isolated.Using Hug′s double-layer soft agar culture and clonal culture on SHZ-88 cell line.Result:The insensitivity of the cell strain to TAM dropped by ten times than that of parent cell line,but has no change of sensitivity to E 2.Conclusion:The establishment of the cell strain can provide a practical tool to study the biological characteristics of resistant breast cancer cells and select the effective anticancer drug for the drug-resistant breast cancer.