甲醛和苯并(a)芘联合作用对DNA损伤的体外研究

Study of formaldehyde and benzo(a)pyrene combination on DNA damage in vitro

以豚鼠肺巨噬细胞为研究对象，研究了甲醛和苯并（ａ）芘（ＢａＰ）在ＬＤ５０、ＤＮＡ－蛋白质交链（ＤＰＣ）、ＤＮＡ单链断裂（ＳＳＢ）等３项指标上的联合作用。结果表明，甲醛和苯并（ａ）芘对豚鼠肺巨噬细胞的ＬＤ５０分别为２４０．４ｍｇ／Ｌ和１３．８ｍｇ／Ｌ，联合使用的ＬＤ５０为６１．７ｍｇ／Ｌ（其中甲醛为５５．８ｍｇ／Ｌ，ＢａＰ为５．８８ｍｇ／Ｌ），呈增强作用。甲醛能引起ＤＰＣ，ＢａＰ不能引起ＤＰＣ，联合作用时甲醛引起的交链程度降低；甲醛和ＢａＰ都能引起ＳＳＢ，联合作用时ＳＳＢ的程度增强。由此可见，甲醛和ＢａＰ对细胞杀伤和ＳＳＢ具有增毒作用。

Alveolar macrophages of guinea pig were used in this study to investigate the effect of formaldehyde(HCHO) and benzo(a)pyrene (BaP) combination on LD50,DNA protein crosslinks (DPC) and DNA single strand breaks (SSB) in vitro.The results showed that LD50 of HCHO was 240 4mg/L,that of BaP was 13 8mg/L,and the combined LD50 was 61 7mg/L(HCHO was 55 82mg/L,BaP was 5 58mg/L).The type of combination was synergetic action.Only HCHO could induce DPC,and the intense of DPC decreased when used with BaP together.Both HCHO and BaP could induce SSB,and this effect increased when the two chemicals used together.So the combination on SSB was synergetic action too.\ \