抑癌基因p^(16)在肝细胞癌及癌旁组织中的分布、表达及意义

DISTRIBUTION, EXPRESSION AND SIGNIFICANCE OF SUPPRESSOR GENE p^(16) IN HUMAN PRIMARY HEPATOCELLULAR CARCINOMA AND ADJACENT LIVER TISSUE

目的 :探讨抑癌基因 p16与原发肝癌 (primaryhepatocellularcarcinoma ,HCC)发生及恶性程度的关系。方法 :p16是一种新的抑癌基因 ,应用免疫组织化学法检测了 6 9例HCC和 6 3例癌旁组织的 p16表达。结果 :p16蛋白免疫组织化学阳性信号为棕黄色颗粒 ,主要分布在胞浆 ,少数细胞核内也可见阳性显色 ,p16蛋白在癌组织及癌旁组织中的阳性率分别为 2 6 5 % (18/6 9)和 92 % (5 8/6 3) ,两者比较差异显著 (P <0 0 1)。 6 9例HCC标本 ,Ⅰ级、Ⅱ级、Ⅲ级中 p16蛋白的检出率分别为 5 2 6 % (10 /19)、 2 3 3 % (7/30 )、5 % (1/2 0 ) ,p16蛋白在高分化HCC中的表达率明显高于低分化HCC组 (P <0 0 5 )。结论 :抑癌基因 p16的表达和分布与HCC发生、发展及恶性程度有密切的关系 。

objective: To study the relationship between the expression of suppressor gene p16 in primary hepatocelluar carcinoma (HCC) and its development and malignancy. Methods: p16 is a new tumor suppressor gene and its expression was detected in 69 specimen of HCC and 63 specimen of HCC adjacent liver tissues by the method of immunohistochemisty. Results: The positive immunocytochemistrical staining of p16 protein was brown-yellow granules locating mainly in cytoplasm whereas with a small number of cells, the positive staining was identified within the nucleolus region. The positive rate of p16 expression was 26.5% (18/69) in HCC samples and 92% (58/63) in HCC adjacent liver tissurs and the difference of the rate between the two groups was significant (P<0.01). In 69 specimen of HCC, p16 protein positive expression among samples of grad Ⅰ, Ⅱ and Ⅲ were 52.6% (10/19), 23.3%(7/30) and 5%(1/20), respectively. p16 protein expression rate of highly differentiated HCC was significantly higher than that in lower differentiated ones (P<0.05). Conclusion: The expression and distribution of the suppresser gene p16 is closely related with the generation, development and malignancy of HCC. It is suggested that the inactivation of p^(16) expression may occur at the early stage of HCC development.