紫外分光光度法测定抗病感口服液中靛玉红的含量

Determination of Indirubin in Kangbingan Oral Liquid by Ultraviolet Spectrophotometry

目的建立紫外分光光度法(UV)测定抗病感口服液中靛玉红含量的方法。方法采用Helios Gamma紫外-可见分光光度计,以靛蓝、靛玉红对照品分别制成2～8μg/mL的氯仿溶液,按处方比例配制无板蓝根阴性空白溶液,在500～700nm波长处分别测定。结果靛玉红在540.0nm处有最大吸收,靛蓝在634.5nm处有最大吸收,在0.20～1.40μg/mL范围内呈良好线性关系(r=0.9994),平均回收率为99.05%,RSD=0.87%(n=6)。结论本方法操作简便,结果准确,可用于抗病感口服液的质量控制。

Objective To determine the content of indirubin in Kangbingan Oral Liquid by UV spectrophotometer methods. Methods The content of indirubin was determined by Heltos Gamma ultravioletvisible spectrophotometer with chloroformic solution of 2-8 μg/mL confected by standard sample of indigotin and indirubin. The sample of negative blank solution was confected by the prescription without Radix Isatidis. The standard sample and the blank sample were determined separately with the wavelength at 500-700 nm. Results Indirubin had the maximum absorption at wavelength of 540.0 nm and indigotin had the maximum absorption at wavelength of 634.5 nm. The linear correlation was available at the range of 0.20-1.40 μg/mL, and the correct coefficient was 0.999 4. The average recovery rate was 99.05% and RSD was 0.87% （n =6）. Conclusion This method is simple and accurate, and can be used to control the quality of Kangbingan Oral Liquid.