板栗内生真菌B_2与病原菌D_1菌株培养条件研究

Study of Culture Conditions on the B_2 Strain of Endophytic Fungi and Pathogenic bacteria D_1 from Castanea millissima

以菌丝体生物量为主要指标,在25℃下以120r/min的摇床速度,通过单因素实验确定了板栗2种树栖真菌液体培养适宜的碳源、氮源、最适pH.研究表明B2的最适碳源是葡萄糖,最适氮源是酵母膏,最适pH是5.0,最适固体培养基是w(木屑)78%、w(麦麸)20%、w(石膏粉)1%、w(蔗糖)1%;病原菌D1的最适碳源是蔗糖,最适氮源是蛋白胨,最适pH是6.2,最适固体培养基为w(木屑)78%、w(麦麸)10%、w(石膏粉)1%、w(蔗粉)1%、w(豆粉)10%.

The test was done at 25 ℃ and 120 r/min on the mycelial biomass.The most suitable carbon source,nitrogen source,the most suitable pH and the most suitable solid medium compounding were determined by the single factor experiments.Experiments showed that the most suitable carbon source for B2 is glucose,the most suitable nitrogen source for B2 is yeast cream,the most suitable pH for B2 is pH 5.0,and the most suitable solid medium compounding for B2 is w（wood powder）78%、w（wheat bran）20%、w（gypsum powder）1%、w（cane sugar）1%.Furthermore experiments showed that the most suitable carbon source for D1 is sucrose,the most suitable nitrogen source for D1 is peptone,the most suitable pH for D1 is pH 6.2,and the most suitable solid medium compounding is w（wood powder）78%、w（wheat bran）10%、w（gypsum powder）1%、w（cane sugar）1%、w（soya Bean powder）10%.