摘要
目的建立四季三黄片的质量控制方法。方法薄层色谱法定性鉴别四季三黄片中大黄，栀子，黄芩，黄柏。HPLC法测定黄芩苷的含量，色谱柱：ODS-2 HYPERSIL（250mm×4．6mm，5μm）；流动相：乙腈-水-磷酸（25：75：0．2）；流速：1．0mL·min^-1；检测波长280nm；柱温25℃；紫外检测波长280nm。结果薄层鉴别专属性强，色谱宽点清晰。黄芩苷在0．02968-0．5935μg范围内呈良好的线性关系，加样平均回收率为98．05％，RSD=0．9946％（n=6）。结论本法简便，可靠，准确，可用于该制剂的质量控制。
OBJECTIVE To establish a method to control the quality of Sijisanhuang Tablets. METHODS The effective ingredients in Radix et Rhizoma Rhei, Fructus Gardeniae, Radix Scutellariae and Cortex Phellodendri Chinenesisi were distinguished by TLC. Baicalin was determined by HPLC. Column ： ODS- 2 HYPERSIL （250mm × 4.6mm, 5μm）, mobile phase： acetonitrile--water-- phosphoric acid （25 ： 75： 0.2）, flow rate： 1.0mL.min^-1 , wavelength： 280nm, column temperature： 25℃ RESULTS TLC method had a good specificity,TLC spectrum was clear. The linear range was 0. 02968 - 0. 5935μg （ r 1. 0000） and recovery rate was 98. 05%, RSD = 0. 9946% （ n = 6）. CONCLUSION This method was simple, reliable, accurate,and can be used to contol the quality of Sijisanhuang Tablets.
出处
《齐鲁药事》
2010年第1期20-22,共3页
qilu pharmaceutical affairs
