β-榄香烯对肝癌细胞增殖及细胞周期的影响

Effect of β-elemene on the proliferation and cell cycle of hepatic carcinoma cell

目的观察β-榄香烯对体外培养肝癌细胞增殖以及细胞周期的影响。方法肝癌7402细胞体外培养,β-榄香烯干预;四甲基偶氮唑蓝(MTT)法检测β-榄香烯对细胞增殖的抑制作用;流式细胞仪检测细胞周期。结果肝癌7402细胞经过β-榄香烯干预,其存活率明显降低,并呈剂量依赖性。细胞周期检测结果显示,β-榄香烯干预后,进入S期和G2期细胞明显减少,G1期细胞增多,细胞周期被阻滞于G1期。结论β-榄香烯可明显抑制肝癌细胞的增殖及细胞周期,阻滞细胞从G1期进入S期。

Objective To study the effect of β-elemene on the proliferation and cell cycle of hepatic carcinoma cells. Methods Hepatic carcinoma 7402 cells were cultured in vitro, and treated with β-elemene for 48 h; MTT assay was used to evaluate the proliferation of 7402 cells; Cell cycle was analyzed by using flow cytometry with PI staining. Results After treated with β-elemene, the proliferation of 7402 ceils was apparently inhibited in a dose-dependent manner; FCM assays showed that cells in S and G2 phases were decreased, while increased in G1 phase, which indicated that G11t phase arrest happened. Conclusion β-elemene can directly influence the proliferation and cell cycle of hepatic carcinoma cells, arrest cells entering S phase from G1 phase.