绿色饲料添加剂在抑菌实验中的研究

Research on Green Feed Additive in Antibacterial Test

使用不同浓度的植酸、咖啡酸、甘氨酸进行配比，采用平板培养基小钢圈滴加药品法考察他们对畜禽致病菌尤其是革兰氏阴性菌的抑制特性。实验结果表明：三种物质都具有协同抑菌作用，对畜禽的霍乱弧菌、大肠杆菌、巴氏杆菌、气单孢菌等革兰氏阴性致病菌均有不同程度的抑制作用。抑菌效果达到中度敏感（抑菌圈直径15mm）时，溶液中的配比：植酸浓度为5％，咖啡酸为0．01％，甘氨酸为0．3％。本研究为开发新型的绿色饲料添加剂以及其广谱的抑菌范围打下了基础。

The use of different concentrations of phytic acid, caffeic acid, glycine for the ra- tio, the use of small steel plate medium dropwise Drugs Act on the livestock and poultry inspec- tion of pathogens, especially gram- negative bacteria inhibiting properties. The experimental results show that all three substances have synergistic inhibitory effect on the livestock of the cholera vibrio, E. coli, Pasteurella, Aeromonas Gram - negative bacteria have different degrees of inhibition. Achieve moderate inhibitory effect of sensitive （inhibition zone diameter of 15mm）, the solution of phytic acid concentration ratio of 5%, 0.01% for caffeic acid, glycine, 0.3% ; of this study was to develop a new type of green feed additives, as well as the scope of its broad - spectrum antibacterial and lay the foundation.