胰岛素受体底物-2基因1057G/D多态性与妊娠期糖尿病的相关性

Association of insulin receptor substrate-2 gene 1057G/D polymorphism with gestational diabetes mellitus

目的研究胰岛素受体底物-2(IRS-2)基因1057G/D多态性在南京地区汉族孕妇中的频率分布及其与妊娠期糖尿病(GDM)患者胰岛素抵抗的相关性。方法采用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)检测110例GDM孕妇(GDM组)及100例正常孕妇(NGT组)IRS-2基因1057G/D等位基因分布及基因频率,同时测定所有研究对象的空腹血糖(FPG)、空腹胰岛素(FIns),并计算胰岛素抵抗指数(HOMA-IR)以评价胰岛素抵抗状态。结果①IRS-2基因1057 D等位基因频率在GDM和NGT组中分别为25.91%和31.50%,差异无显著意义(P>0.05);②与NGT组相比,GDM组的FPG、FIns和HOMA-IR明显升高,差异有显著意义(P<0.05)。③GDM组和NGT组中不同基因型孕妇FPG、FIns和HOMA-IR等差异均无显著性(P>0.05)。结论南京地区汉族孕妇中IRS-2基因1057G/D多态性可能与妊娠期糖尿病的发生和胰岛素抵抗状态无关。上述结果尚待进一步通过联合检测和扩大样本量来确认。

Objective To investigate the frequency of insulin receptor substrate-2 （IRS-2） gene 1057G/D polymorphism in pregnant women of Chinese HaM population in Nanjing and its effects on insulin resistance in women with gestational diabetes mellitus （GDM）. Methods The genotypes of IRS-2 1057G/D polymorphism were determined in 110 GDM patients and 100 healthy pregnant women by PCR-restriction fragment length polymorphism（RFLP）. Serum levels of glucose and insulin were estimated by biochemical methods. Homeostasis model assessment-insulin resistance index（HOMA-IR） was applied to assess the status of insulin resistance. Results （1） The frequency of allele D was 25.91% in GDM group and 31.50% in control group（ P 〉 0.05 ） ; （2） Comparing with the control group, GDM group had higher levels of FPG, FINS, and HOMA-IR （ P 〈 0.05 ） ; （3） There was no significant difference in the levels of FPG, FINS and HOMA-IR among three groups with different genotypes in either case or control group. Conclusions IRS-2 1057G/D polymorphism may not be associated with increased susceptibility to GDM in pregnant women of Chinese HaM population.