健康人群肿瘤坏死因子-α基因多态性的分析

Analysis on polymorphisms of TNF-α gene in healthy population

了解汉族健康人群中TNF-A基因多态性的分布,研究TNF-α表达与相关疾病之间的联系。采用PCR-限制性长度片段多态分析法检测140名重庆地区汉族健康人群的TNF-A-308,TNF-A-857位点基因多态性,计算其基因型和等位基因频率,结果显示TNF-A-308 G/G、G/A、A/A基因型的频率分别为89%、11%、1%,其等位基因的发生频率以G等位基因最常见(93%),其次为A等位基因(7%)。TNF-A-857 C/C、C/T、T/T基因型的频率分别为68%、36%、8%,其等位基因发生频率以C等位基因最常见(81%),其次为T等位基因(19%)。由结果可以得出重庆地区汉族健康人群TNF-A-308位点存在G/A多态性,TNF-A-857位点存在C/T多态性。

To observe the polymorphisms of TNF-α genes in Han healthy people and establish the base on relationship between the expressions of TNF-α and certain disease, gene polymorphisms of TNF-A-308 and TNF-A-857 were genotyped by the PCR-RFLP method. The genotypes and allele frequencies were calculated and analyzed. In Han population, the frequencies of three TNF-A-308 genotype of G/G, G/A and A/A were 89% , 11% and 1% , respectively, and the frequencies of allele G （93%） were most commonly seen followed by the frequencies of allele A （7%）. The frequencies of TNF-A-857 genotype of C/C, C/T and T/T were 68% , 36% and 8% , respectively, and the frequencies of allele C （81%） was most commonly seen followed by the frequencies of allele T （ 19% ）. It indicated that among the Han population in Chongqing, the TNF-A-308 site exists G/A polymorphism. In addition, the TNF-A-857 site exists C/T polymorphism.