人脐血造血细胞体外扩增与表面标志的关系

Relation Ship Between Human Umbilical Cord Blood Hematopoietic Cells Expanding in Vitro and Cell Surface Markers in Long-term Culture

目的：对脐血造血细胞体外扩增及移植的最佳时机选择进行探讨。方法：使用SCF，rIL－3，rIL－1β和rlL－6长期培养人脐血造血细胞，观察其体外扩增与表面标志的变化。结果：培养14天细胞增殖达高峰，28天下降，但仍为第7天的29．88倍。CD细胞第14天达高峰，28天后仍维持一定水平。HLA－DR+细胞培养14～21天达高峰，第35天仍较高。CD细胞培养第7天显著增多，14～21天达高峰，28天后明显下降。结论：此培养体系能够促进脐血造血细胞增殖、扩增，并维持其存活。14天造血细胞数量最多，状态最佳，适于移植。

Hematopoietic cells of human umbilical cord blood were cultured with SCF, rIL-3,rIL-1β and rIL-6 in vitro for long-term, and their expansion and change of cell suface markers were observed, Hematopoietic cell proliferation reached the highest peak in the fourteenth day. It dropped in the twenty-eighth day but still was higher for 29.88 times than in the seventh day. CD34 positive cells reached the highest peak in the fourteenth day,and still maintained in relative high level after the twenty-eighth day. HLA-DR positive cells reached the highest peak during the culture of the fourteenth to twenty-first days and still were higher in the thirty-fifth day. CD33 positive cells increased markedly in the seventh day of culture,reached the highest peak during the culture of fourteenth to twenty-first days and dropped markedly after the twenty-eighth day. The results suggest that the culture system is able to promote prolifearation and expansion of human umbilical cord blood hematopoietic cells,and maintains them survivance. In the fourteenth day,the quantity of hematopoietic cells is the most,vitality is optimal and it is suitable for transplantation.