核重编程机制的影响因素

Influencing factors of the mechanism of nuclear reprogramming

哺乳动物体细胞核移植技术在农业、生物技术、医药生产和濒危动物保护等方面具有很大的潜力和应用价值,已成为目前发育生物学研究的重要方法。但是核重编程仍是核移植技术的关键因素,制约了重构胚胎干细胞的研究。只有供核发生完全重编程,重构胚胎才能正常发育。核重编程与供核者的年龄,供核细胞的组织来源、分化状态、细胞周期、传代次数,供核细胞的表观遗传标记以及供卵者的年龄、卵子的成熟度等因素有关。创造各种适于核重编程的条件有利于从更高的起点开展核移植胚胎干细胞的研究,提高重构胚胎干细胞建系效率。

Mammal somatic cell nuclear transfer（SCNT） technology has very big potential and application value in agriculture, biological technology, medicine production and endanger animal. At present, SCNT has become an important method in developmental biology research. However, nuclear reprogramming standing in the way of reconstructed embryo stem cell research is still an important factor in the procedure of nuclear transfer and has a predominant effect on reconstructed embryo development. There are many influencing factors influencing on nuclear reprogramming, such as the age of somatic cell donor, the source of somatic cell and its differentiation, cycle stage, passage number and epigenetic marks, the maturation of oocytes and the donor＇s age etc. Anyway, setting up a smart situation for nuclear reprogramming will improve the efficiency of producing SCNT-ES cell line and benefit the therapeutic cloning.