血浆D二聚体和GMP-140测定在DIC诊断中的意义

The Diagnostic Significance of Examining the Plasma Levels of D-dimer and GMP-140 in Disseminated Intravascular Coagulation (DIC)

目的：探讨分子标志物在弥散性血管内凝血（DIC）诊断中的价值。方法：分别检测DIC患者20例（DIC组）、DIC疑诊组11例（DIC疑诊者）和正常人10例（正常对照组）血浆D二聚体和血小板颗粒膜蛋白（GMP－140）水平。结果：以上三组血浆D二聚体水平分别为4．43±3．43，2．03±0．17，0．36±0．17（mg／L）。血浆GMP－140水平分别为53．78±27．55，33．32±16．50，17．29±4．84（ng／ml）。DIC组血浆D二聚体和GMP－140显著高于DIC疑诊组和正常对照组，而DIC疑诊组与正常对照组差异尤显著性。动态观察发现，血浆D二聚体升高的6例DIC疑诊者有3例随后转变成DIC。结论：血浆D二聚体和GMP－140是DIC时敏感指标，监测二指标有助于DIC的早期诊断。

In order to demonstrate the diagnostic value of molecular markers in disseminated intravascular coagulation syndrome (DIC),plasma samples from 20 patients with DIC (DIC group), 11 patients with suspected DIC(suspected DIC group) and 10 normal controls(control group) were examined for D-dimer and platelet granule membrane protein (GMP-140) by using ELISA. The results showed that the plasma levels of the three groups were 4.43±3.43, 2.03±1.72, 0.36±0.17 (mg/L) in D-dimer and 53.78±27.55, 33.32±16.50, 17.29±4.84(ng/mI) in GMP-140 respectively. The plasma concentrations of the two molecular markers in DIC group were much higher than those in suspected DIC group or control group. In contrast,there were no significant differences between suspected DIC group and normal controls. The results also showed that 3 of 6 suspected DIC cases which had higher plasma levels of D-dimer became to be DIC patients when observed dyanamically. In conclusion,the indexes of GMP-140 and D-dimer are sensitive. It is helpful to diagnose DIC during early stage by examining the two markers.