摘要
选用四种离子交换剂对玻璃酸进行分离纯化研究。结果表明，采用D315大孔离子交换剂，以去离子水为淋洗液、0．6mol／LNaCl溶液为洗脱液，纯化效果较佳。纯化产品杂蛋白含量≤0．3％，粘均分子量＞96×104。
The optimal purification conditions of HA by ion-exchange chromatography were studied.As a result, macroporous ion-exchange rein D3l5 and 0. 6 mol/L NaCl solution as a eluent wereselected. The purification efficiency was very well. The content of protein was less than 0. 3 %. Theaverage molecular weight of HA was more than 960.000.
出处
《中国生化药物杂志》
CAS
CSCD
1998年第5期240-242,共3页
Chinese Journal of Biochemical Pharmaceutics
关键词
离子交换层析
玻璃酸
纯化
生化药物
Hyaluronic acid, Ion-exchange chromatography, Purification
