胶质母细胞瘤放疗前后免疫相关基因差异表达及意义

Differential Expression of Immune-associated Gene of Glioblastoma Tissue before and after Radiotherapy with 60Gy and Its Significance

目的:研究胶质母细胞瘤(GBM)组织照射60 Gy后基因表达谱中免疫相关基因表达差异的变化及意义。方法:应用含13 929条人类全长基因cDNA表达谱芯片BioStarH-141s(2004)对两例胶质母细胞瘤组织进行放疗前及放疗60 Gy后检测,并分析它们之间的基因表达差异。结果:人脑胶质母细胞瘤放射治疗60 Gy后与放射治疗前比较,表达差异基因中改变最明显的功能群是免疫系统相关的基因,如IGLV1-44、SLPI、CXCL14等上调。细胞增殖、细胞调亡、细胞周期、DNA修复系统也有部分基因发生明显变化,如MLL5上调、POLR2B下调等。结论:提示对胶质母细胞瘤组织照射60 Gy后基因表达谱改变的研究可以更好地阐明放射敏感性差异机理,为放疗前或放疗早期寻找到预测放射敏感性分子标志提供理论依据。

Objective：To study the change of gene express profile of glioblastoma（GBM） tissue before and after the radiation with 60 Gy and analyze the correlation among the gene. Method： Genechip BioStarH-141s（2004） profile which contained 13929 points of full length human genes was used to detect two GBM tissue before and after radiation and to analyse the genes expressed differently. Result：The results showed that, after radiation with 60Gy, there were 8 up-regulate genes and 10 down regulate genes. The gene group which changed the most obviously was related to the function of immunity. Some genes related to cell proliferation,apoptosis,cell cycle and DNA-repair system, also differently expressed in evidence, for instance, up regulate gene： MLL5 and down-regulate gene： POLR2B. Conclusion：To research the change of the gene express profile of GBM tissue before and after the radiation can better clarify the mechanism of radiation sensitivity. It may find molecular markers to predict radiation sensitivity before or at the forepart of radiation therapy.