8-溴-7-甲氧基白杨素对人小细胞肺癌细胞增殖和凋亡的影响

Effect of 8-bromo-7-methoxychrysin(BrMChR)on proliferation and inducing apoptosis in human small cell lung cancer cell line

目的观察8-溴-7-甲氧基白杨素(BrMChR)对人类小细胞肺癌(SCLC)细胞株NCI-H446增殖和凋亡的影响。方法体外培养NCI-H446细胞,MTT比色法测定BrMChR对NCI-H446细胞增殖的抑制作用,平皿克隆形成法测定BrM-ChR对NCI-H446细胞集落形成的抑制作用;DNA凝胶电泳观察BrMChR诱导基因DNA梯形条带,流式细胞术(FCM)分析BrMChR对NCI-H446细胞周期的影响。结果MTT法显示BrMChR能明显抑制NCI-H446细胞增殖,且呈浓度依赖性;平皿克隆形成法结果显示BrMChR能明显抑制NCI-H446细胞集落的形成;琼脂糖凝胶电泳检测呈现典型"梯形"DNA条带;FCM结果显示BrMChR将NCI-H446细胞周期阻滞在G1期。结论BrMChR具有抑制NCI-H446细胞增殖并诱导其凋亡的作用。

Objective To observe the effect of 8-bromo-7-methoxychrysin（BrMChR） on proliferation and inducing apoptosis in human small cell lung cancer（SCLC） cell line NCI-H446. Methods The inhibitive effect of method was used to test inhibition effect of BrMChR on clone formation of NCI-H446 cells. DNA ladder bands were observed by DNA agarose gel electrophoresis. Cell cycle phase was detected by flow cytometry（FCM） with PI staining. Results MrlT test showed the proliferation of NCI-H446 cell was strongly inhibited by BrMChR in a dose-dependent manner. BrMChR could inhibit clone formation of NCI-H446 cells. DNA fragment assay indicated that DNA ladder bands could be shown in DNA agarose gel electrophoresis after treatment with BrMChR of different concentration for 48h. FCM analysis indicated cell cycle phase was blocked by BrMChR in G1 phase. Conclusion BrMChR can inhibit the proliferation of NCI-H446 cells and induce apoptosis.