摘要
目的探讨胰腺癌组织中p53和Bcl-2家族(Bcl-2、Bax、Bcl-xL、Bcl-xS)蛋白的表达及其与细胞凋亡的关系。方法免疫组化法检测35例胰腺癌组织中p53蛋白表达,分为p53阴性表达组(18例)和p53阳性表达组(17例);Westernblot法检测胰腺癌组织中p53、Bcl-2、Bax、Bcl-xL和Bcl-xS蛋白表达;TUNEL法检测35例胰腺癌组织中凋亡指数(AI)。结果Bcl-2蛋白在p53阳性表达组上调,而在p53阴性表达组下调(P=0.047);Bax和Bcl-xL蛋白在p53不同表达两组中都明显上调(P=0.274,P=0.334);Bcl-xS在p53阳性表达组明显下调,在p53阴性表达组明显上调(P=0.01);在p53不同表达两组中,AI分别为12.1±2.47和9.1±1.48(P=0.023);上述Bcl-2家族各成员表达与AI无相关性(P>0.05),而p53不同表达Bcl-2/Bax比值与AI有明显的相关性(P<0.01)。结论Bcl-2是依赖p53调节的抗凋亡蛋白,Bcl-xS是依赖p53调节的促凋亡蛋白,而p53主要通过调节Bcl-2/Bax比值发挥凋亡调节作用。
Objective The biological behavior of ductal adenocarcinoma of the pancreas is, in part, relevant to apoptosis, which is closely related to p53 and bel-2 family protein. A systematic investigation of the apoptosis-related bcl-2 family modulated by p53 in pancreatic cancer is lacking. The purpose of this study was to clarify the association between p53 and bel-2 family（ Bel-2, Bax, Bcl-xL, Bcl-xS） expression in pancreatic cancer. Methods A total of thirty-five pancreatic cancer patients were studied. The expression of p53 protein in pancreatic cancer was assessed using the immunohistochemieal method, which categorized the pancreatic cancer patients into two groups： group 1, with immunonegative p53 （18 cases）; and group 2, with immunopositive p53 （17 cases）. The expression of p53, Bcl-2, Bax, Bcl-xL, and Bel-xS in the 35 pancreatic cancer eases detected by Western blotting was quantified with a densitometer. The apoptosis of the 35 pancreatic cancer eases was determined by terminal deoxynucleotidyltransferase-mediated UTP end-labeling（TUNEL）. Results Bcl-2 was remarkably up-regulated in group 2（ 15 of 17 ）, but was down-regulated in group 1 （5 of 18 ）. Bax was up-regulated in both groupl （ 15 of 18 ） and group 2 （ 15 of 17 ）. Bcl-xL was up-regulated in both group 1 （ 14 of 18 ） and group 2 （ 15 of 17 ）. Bcl-xS was remarkably down-regulated in group 2 compared to group l （P = 0. 01 ）. The apoptosis indexes of groups 1 and group 2 were 12. 1 ± 2. 47 and 9. 1 ± 1.48, respectively（ P = 0. 023 ） ,there was no relationship between AI and expression of Bel- 2, Bax, Bcl-xL and Bcl-xS, respectively（ P 〉0. 05 ）. AI was remarkably increased in high Bcl-2/Bax ratio group, and low in Bcl-2/ Bax ratio group（ P = 0. 012）. Conclusion Bcl-2 and Bel-xS represented the significant anti- and proapoptotic proteins, respectively, modulated through a p53-dependent pathway in pancreatic eancer, and p53 modulated apoptosis mainly through bcl-2/bax ratio.
出处
《临床肿瘤学杂志》
CAS
2010年第1期30-33,共4页
Chinese Clinical Oncology
基金
海南省自然科学基金资助项目(809043)
