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利用siRNA抑制环氧合酶-2对人胃癌裸鼠移植瘤的影响 被引量：4

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摘要 目的观察环氧合酶2(COX-2)对人胃癌裸鼠移植瘤的影响并探讨其机制。方法构建靶向COX-2表达质粒和在BALB/c裸鼠皮下建立SGC-7901人胃癌细胞动物模型,用COX-2表达质粒基因转染治疗。结果构建的COX-2表达质粒在体内、外均可稳定表达。COX-2基因治疗可抑制裸鼠皮下肿瘤的生长和淋巴管的生成。COX-2基因转染下调血管内皮生长因子-C(VEGF-C)的表达。结论通过构建靶向COX-2 siRNA真核表达载体可抑制人胃癌裸鼠移植瘤的生长和淋巴管生成,COX-2对抑制胃癌细胞(SGC-7901)治疗有确切效果。
作者 黄国民 林瑞新 房学东 刘斌
机构地区 吉林大学第二医院普外科
出处 《中国老年学杂志》 CAS CSCD 北大核心 2010年第2期210-212,共3页 Chinese Journal of Gerontology
关键词 胃肿瘤 环氧合酶-2 基因治疗
分类号 R735.2 [医药卫生—肿瘤]
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参考文献7

  • 1Corcoran CA,He Q, Huang Y,et al. Cyclooxygenase-2 interacts with p53 and interferes with p53-dependent transcription and apoptosis[J]. Oncogene ,2005 ;24- : 1634-40.
  • 2Yamagishi M, Noda M, Tatsumi Y, et al. Correlation between cyclooxygenase-2, proliferative activity, and mucin phenotype in human advanced gastric cancer[J]. J Gastroentero1,2004 ;39 : 1143-9.
  • 3Tang RF, Wang SX, Peng L, et al. Expression of vascular endothelial growth tactors A and C in human pancreatic cancer[J].World J Gastroenterol,2006 ; 12 ( 2 ) :280-6.
  • 4Yonemura Y,Endo Y,Tabata K,et al. Role of VEGF-C and VEGF-D in lymphangiogenesis in gastric cancer[J].Int J Clin 0ncol,2005;10(5 ) : 318-27.
  • 5Shayan R, Achen MG, Steven A, et al. Lmphatic vessels in cancer metastasis : bridgingthegaps [ J]. Carcinogenesis ,2006 ;27 ( 9 ) : 1729-38.
  • 6Chen Z, Vamey ML, Baekora MW, et al. Down-regulation of vascular en- dothelial cell gowth factor-C expression using small interfering RNA vec- tors in mammary tumors inhibits tumor lymphangiogenesis and spontaneous metastasis and enhances survival [J]. Cancer Res, 2005 ; 65 ( 19 ) :9004-11.
  • 7黄国民,房学东,丁相福,姜洪伟.环氧合酶-2和血管内皮生长因子-C的表达与胃癌淋巴结转移的关系[J].中国老年学杂志,2008,28(16):1627-1628. 被引量：4

二级参考文献6

  • 1Costa C,Soares R, Reis-Filho JS,et al. Cyclooxygenase-2 expression is associated with angiogenesis and lymph node metastasis in human breast cancer[J]. Clin Pathol,2002 ;55:429-34.
  • 2Zha S, Yegnasubramanian V, Nelson WG, et al. Cyclooxygenase-2 in cancer progress and perspective[J]. Cancer Left ,2004 ;215 ( 1 ) : 1-20.
  • 3Karkainen MJ, Makinen T, Alitalo K, et al. Lymphatic endothelium : a new frontier of metastasis research[J]. Nat Cell Biol,2002 ;4 (1) :2-5.
  • 4Yoncmura Y, Fushids S, Bando E,et al.Lymphangiogenesis and the vascular endothelial growth receptor (VEGFR)-3 in gastric cancer[J]. Eur J Cancer,2001 ;37(&) :918-23.
  • 5Su JL,Shih JY,Yen ML,et al. Cyclooxygenase-2 induces EPI-and HER- 2/Neu-dependent vascular endothelial growth factor-C up-regulation: a novel mechanism of lymphangiogenesis in lung adenocarcinoma [J]. Cancer Res ,2004 ;64( 1 ) :554-64.
  • 6Saukkonen K, Rintahaka J, Sirula A, et al. Cyclooxygenase-2 and gastric carcinogenesis[J]. APMIS ,2003 ; 111:915-25.

共引文献3

同被引文献71

  • 1高福莲,王峰,吴景兰,乐晓萍,张钦宪.靶向人胃癌SGC7901/VCR细胞多药耐药基因1小分子干扰RNA的有效序列筛选[J].中华肿瘤杂志,2006,28(3):178-182. 被引量：10
  • 2何英,唐利立.siRNA真核表达载体的构建及其对CXCR4基因的沉默效应[J].中国普通外科杂志,2006,15(10):736-740. 被引量：5
  • 3Wigle JT,Oliver G.Prox1 function is required for the development of the murine lymphatic system[J].Cell,1999,98(6):769-778.
  • 4Karkkainen MJ,Haiko P,Sainio K,et al.Vascular endothelial growth factor C is required for sprouting of the first lymphatic vessels from embryonic veins[J].Nat Immunol,2004,5(1):74-80.
  • 5Francois M,Caprini A,Hosking B,et al.Sox18 induces development of the lymphatic vasculature in mice[J].Nature,2008,456(7222):643-647.
  • 6Kazenwadel J,Michael MZ,Harvey NL.Prox1 expression is negatively regulated by miR-181 in endothelial cells[J].Blood,2010,116(13):2395-2401.
  • 7Pedrioli DM,Karpanen T,Dabouras V,et al.miR-31 functions as a negative regulator of lymphatic vascular lineage-specific differentiation in vitro and vascular development in vivo[J].Mol Cell Biol,2010,30(14):3620-3634.
  • 8Braun M,Flucke U,Debald M,et al.Detection of lymphovascular invasion in early breast Cancer by D2-40(podoplanin):a clinically useful predictor for axillary lymph node metastases[J].Breast Cancer Res Treat,2008,112(3):503-511.
  • 9Wang XL,Fang JP,Tang RY,et al.Different significance between intratumoral and peritumoral lymphatic vessel density in gastric cancer:a retrospective study of 123 cases[J].BMC Cancer,2010(10):299.
  • 10Karatzanis AD,Koudounarakis E,Papadakis I,et al.Molecular pathways of lymphangiogenesis and lymph node metastasis in head and neck Cancer[J].Eur Arch Otorhinolaryngol,2012,269(3):731-737.

引证文献4

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中国老年学杂志
2010年 第2期

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