尾吊、噪声及其复合因素对大鼠免疫功能的影响

Effects of Tail-suspension,Noise and Combination of Two Stresses on Immune Function of Rats

目的研究尾吊、噪声以及两种因素复合对大鼠免疫功能的影响。方法大鼠30°尾吊21 d、噪声2h及尾吊(21 d)+噪声(2 h)复合处理后,测定大鼠体重、胸腺重、脾重、白细胞及其分类,流式细胞术、Griess法分别测定T淋巴细胞亚群和血清NO含量。结果与对照组相比,尾吊组与尾吊+噪声复合应激组大鼠体重显著下降(P<0.01),外周血白细胞数、中性粒细胞数及其百分比显著增加(P<0.01),而淋巴细胞百分比显著下降(P<0.05),尾吊+噪声复合应激组大鼠T淋巴细胞数和Th亚群数增加(P<0.05),Th/Tc比显著升高(P<0.01),各处理组大鼠血清NO含量均显著降低(P<0.01)。结论尾吊明显影响大鼠体重、外周血免疫细胞含量和分布以及血清NO含量,噪声对尾吊大鼠淋巴细胞亚群的变化有协同效应。

Objective To investigate the effect of tail-suspension,noise stress and combination of them on immune function in rats.Methods The rats were divided into four different groups,A： control;B： noise stress（2 h）;C： tail-suspension（30°,21 d）;D： tail-suspension added noise stress.The body weight,thymus weight and spleen weight,circulating leukocytes and its distribution,T lymphocyte subpopulations（with flow cytometry） and NO level in serum（with Griess method） of rats were determined after exposed to 21 d tail-suspension,2 h noise stress and combination of them.Results Compared with group A,the body weight were significantly decreased（P〈0.01）,the percent of lymphocytes were also reduced（P〈0.05）,but the numbers and percents of circulating leukocytes,neutrophils were markedly increased（P〈0.01） in group C and D.T lymphocyte,Th subsets（both P〈0.05） and Th/Tc ratio（P〈0.01） increased in group D.The NO level in serum was markedly reduced（P〈0.01） in B,C and D groups.Conclusion Tail-suspension had significant effects on the body weight,circulating leukocyte and its distribution,NO level in serum of rats.Noise stress had synergistic effect with tail-suspension on lymphocyte subsets.