条斑紫菜藻红、藻蓝蛋白逐级放大的纯化工艺

The Establishment of Scale-up Isolation Procedure of Phycoerythrin and Phycocyanin from Porphyra yezoensis

采用"破碎-盐析-层析"的方法纯化条斑紫菜藻胆蛋白,并在提取规模上逐步放大。首先在综合比较凝胶层析去盐效率后,从Sephadex G-25、G-100、S-300和CL-6B中选择G-25作为实验流程中的去盐填料,其次将提取流程的初试原料条斑紫菜量逐步放大,选取了1g、20g和400g三个量,结果表明随着初试紫菜量逐步放大,最终所得藻胆蛋白中吸收光谱纯度>3.2的蛋白产率依次提高,其中400g冻干紫菜的藻红蛋白产率为0.323%,藻蓝蛋白产率为0.148%。由此认为该实验工艺流程具有规模放大的潜力,这为高纯度藻胆蛋白的规模生产提供了一条可行的方案。

The way of ＂extracting-salting-chromatography＂ was used to purify the phycoerythrin and phycocyanin from Porphyra yezoensis in process scale-up.First,by comprehensive comparison of efficiency,the Sephadex G-25 was selected from four resins （Sephadex G-25、G-100、S-300 and CL-6B） as the best choice used in crude extract desalting of phycobiliprotein.Then the preparation process of phycobiliprotein was scaled-up with raw material（Porphyra yezoensis） increased from 1g to 20g,and finally to 400g.The results indicated that the yields of purified phycoerythrin and phycocyanin （absorption spectra purity above 3.2） increased during according to process scale-up,with 0.323% phycoerythrin and 0.148% phycocyanin obtained from 400g frozen Porphyra yezoensis blades respectively.It is no doubt that the process involved in the experiment is a potential way for large scale preparation of phycobiliproteins of high purity.