摘要
本文研究了巨噬细胞集落刺激因子(M-CSF)刺激体外培养的正常人外周血单核细胞诱生TNF-α、IL-6和IL-8,检测用培养上清ELISA法。结果显示:M-CSF单独刺激单核细胞产生的TNF-α效价较低,而与LPS+TPA联合刺激则产生高效价的TNF-α,动态观察中,1~7天有渐降的趋势。M-CSF对IL-6。
The purpose of this study was to find out the relationship between M-CSF stimulation and induction of IL-6,IL-8 and TNF-α on human blood monocytes. Stimulating culture with M-CSF was used for cytokine induction and ELISA was used for assays of cytokines in culture supernatants. The results showed that IL-6 and IL-8 were both induced with steadily increasing tendency over 1~4 days. In TNF-αinduction, M-CSF appeared to be a weak stimulant, whereas high level of TNF-α was obtained after combined application of M-CSF+LPS+TPA.There was decreasing tendency over 1~7 days.
出处
《镇江医学院学报》
1998年第3期301-302,305,共3页
Journal of Zhenjiang Medical College