EGF+61基因多态性与肝硬化的相关性研究

Relationship between polymorphism of epidermal growth factor 5' UTR variant G61A gene and cirrhosis in Chinese

目的探讨表皮生长因子(epidermal growth factor,EGF)G61A位点(EGF+61)多态性与肝硬化发生、发展的关系。方法运用多聚酶链式反应-限制性片段长度多态性(PCR-RFLP)技术,检测中国人群中147例肝硬化患者和227例正常对照者中EGF+61位点的基因型,分析其基因型及等位基因的分布频率。结果EGF+61位点AA基因型及A等位基因在正常对照者中的分布频率均高于肝硬化患者(7.0%vs6.1%,30.4%vs27.2%),但其差异均无统计学意义(P>0.05)。EGF+61位点AA基因型及A等位基因在不同的Child-Pugh分级的肝硬化中的分布频率及规律不一,差异均无统计学意义(P>0.05)。结论在中国人群中,EGF+61位点的基因多态性与肝硬化的发生和发展无密切相关性。

Objective To explore the relationship between polymorphism of epidermal growth factor 5＇UTR variant G61A gene（EGF＋61） and the occurrence as well as progression of cirrhosis in Chinese.Methods EGF＋61 polymorphism was analyzed among 147 cirrhosis patients and 227 healthy controls by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） to evaluate the distribution frequency of the genotype and allele.Results The distribution frequency of AA genotype and A allele frequency of EGF＋61 polymorphism were both higher in healthy controls than those in cirrhosis patients（7.0% vs 6.1%,30.4% vs 27.2%）,but there was no significant difference in the distribution（P0.05）.The distribution frequency of AA genotype and A allele frequency of EGF＋61 polymorphism were different in all kinds of Child-pugh grades of cirrhosis,and there was still no significant difference in the distribution（P0.05）.Conclusion The polymorphism of EGF＋61 shows no close relationship to the occurrence and progression of cirrhosis in Chinese.