吐温-80与羟丙基-β-环糊精助溶丹皮酚的超滤适用性研究

Applicability of Ultrafiltration of Paeonol Solubilized with Tween-80 and Hydroxypropyl-β-cyclodextrin

目的研究吐温-80与羟丙基-β-环糊精(HP-β-CD)助溶丹皮酚的超滤适用性。方法以压力、温度、浓度及膜孔径4因素进行正交设计,成分含量为检测指标,测定超滤前后药液成分浓度,通过计算透过率来观察吐温-80及HP-β-CD助溶丹皮酚前后的含量变化。结果压力、温度、浓度及膜孔径4个考察因素对HP-β-CD的透过率无显著影响,溶液浓度对吐温-80透过率有极显著影响(P<0.01),考察因素对二者助溶丹皮酚的透过率无显著影响,但吐温-80助溶的丹皮酚透过率较低。结论HP-β-CD助溶类似难溶性有效成分进行超滤的适用性较好,而吐温-80不适于助溶难溶性有效成分进行超滤。

Objective To study the applicability of nltrafiltration of Paeonol solubilized by tween-80 and hydroxypropyl-β-eyelodextrin. Methods Temperature, pressure, concentration and membrane pore size were selected as factors for orthogonal design method. The concentration was determined in medical solution before and after ultrafiltration, and the transmittance rate（TS） was calculated to observe the content variation of Paeonol solubilized by tween-80 and HP-β-CD. Results Concentration had markedly influence on TS of tween-80（ P 〈 0.01 ） ; the four factors had no significantly impact on TS of, HP-β-CD, but TS of Paeonol solubilized by tween-80 was lower. Conclusion The applicability of uhrafihratiou of unsoluble active components dissolved by HP-β-CD is good, while tween-80 is not suitable for assisting ultrafihration of less soluble components.