亚慢性铝中毒对雏鸡大脑神经细胞凋亡的影响

Effects of Subchronic Aluminium Poisoning on Cerebrum Nerve Cell Apoptosis of Chicken

采用连续腹腔注射固定体积、不同浓度梯度三氯化铝(AlCl3)水溶液法,建立不同程度的亚慢性铝中毒雏鸡模型,通过吖啶橙/溴化乙锭双荧光染色法、琼脂糖凝胶电泳法和流式细胞术对雏鸡大脑神经细胞凋亡进行观测。结果表明:①荧光显微镜下可见,染铝组雏鸡大脑神经细胞发生典型的凋亡和坏死变化,细胞凋亡指数增加,极显著高于对照组(P<0.01),且随AlCl3浓度增加而加重。②染铝组雏鸡大脑神经细胞G0/G1期细胞数量增加,G2/M和S期细胞数量减少,细胞增殖指数降低,细胞凋亡率升高,且随染铝剂量的增多而加重,与对照组比,组间差异均极显著(P<0.01)。③各染铝组雏鸡大脑琼脂糖凝胶电泳检测出现典型的DNA梯形带。上述结果说明,铝可引发雏鸡大脑神经细胞DNA损伤,抑制其增殖,诱导其凋亡。

The chicken models with different levels of subchronic aluminium poisoning were estabolished by continuous peritoneal injection of fixed volume and different concentration gradient aluminium triehloride. The apoptosis of cerebrum nerve cells were detected by agarose gel electrophoresis,AO/EB double fluorescent staining and flow cytometry. The results showed that：（1）The typical changes of apoptosis and necrosis were observed in the fluorescence microscope,and the apoptotic index in the experimental groups was higher than the control（P〈0.01 ）,which inceased along with AlCl3 concentration. （2）The number of cell in GdG1 phase increased,but deceased in G2/M period and S period,and the proliferation index decreased and apoptosis rate increased,which were consist with the concentration of AlCl3,and were signficantly different with the control ones （P〈0.01）. （3）The typical DNA Ladder was detected by agarose gel eleetrophoresis. The results indicated that aluminium could cause the DNA damage,inhibit the proliferation and induce the apoptosis of cerebrum cell.