天然化合物蛇床子素在黄瓜和土壤中残留的高效液相色谱分析

Determination of Natural Compound Osthol in Cucumber and Soi1 by HPLC

建立了蛇床子素在黄瓜和土壤中的残留分析方法。样品经甲醇和丙酮提取,石油醚液–液分配,固相萃取小柱净化后,进行高效液相色谱分析。仪器最小检出量(LOD)为0.2ng,在黄瓜和土壤中的最低检出质量分数(LOQ)均为0.02mg/kg。蛇床子素在黄瓜中的平均回收率为88.80%～93.61%,变异系数为1.94%～4.92%;在土壤中的平均回收率为90.74%～94.55%,变异系数为3.20%～6.45%。

A method for analysis of osthol residues was developed, which used methanol and acetone to extract osthol in the cucumber and soil, followed by liquid - liquid partition with petroleum ether, and purified with SPE, utilizing HPLC to quantify the extract. The limit of detection （LOD） was 0.2 ng, while the quantification limit （LOQ） was 0.02 mg/kg. The average recoveries and coefficients of variation of the method were 88.80% - 93.61%, and 1.94% - 4.92% for cucumber samples, 90.74% - 94.55%, and 3.20% - 6.45% for soil sample, respectively.