丹参水提液对肺癌A549细胞增殖的抑制作用

Inhibitory Effects of Radix Salviae Miltiorrhiae. Water Extract on A549 Cells in Vitro

目的:探讨不同浓度的丹参水提液对体外培养的人肺癌细胞系A549细胞增殖及凋亡的影响。方法:不同浓度的丹参水提液作用于人肺癌细胞A549细胞株24,48,72h后,MTT法观察药物对肺癌A549细胞生长的抑制效应;作用于细胞48h后倒置显微镜观察细胞形态学的改变;应用AO/EB荧光染色法观察不同浓度丹参水提液作用细胞48h后对细胞凋亡的影响;AnnexinV/PI双染流式细胞仪检测经药物作用24h后A549细胞凋亡情况。结果:丹参水提液可剂量依赖性地抑制A549细胞的增殖,抑制增殖作用以48h效果最佳;通过倒置显微镜可观察到细胞的形态学改变;细胞荧光染色出现了明显的凋亡细胞,表现为细胞皱缩、核染色质浓缩、核碎裂等凋亡特征性的形态学改变;流式细胞仪检测结果显示丹参水提液可诱导细胞凋亡。结论:丹参水提液可对人肺癌细胞系A549细胞具有显著的体外增殖抑制作用,其主要作用可能与诱导细胞凋亡有关。

Objective：To explore the effects of Radix Salviae Mihiorrhiae.water extract on proliferation and apoptosis of human lung cancer A549 cell line.Methods： MTT assay was used to observe the anti-proliferation effect of Radix Salviae Mihiorrhiae.water extract on A549 cells for 24 h,48 h,72 h;Morphological changes were observed under optical microscopy ;AO/ EB fluorescence double-dye technology was performed to observe the apoptosis of cells treated for 48 h in fluorescence microscope;The apoptosis rates of A549 cells treated by Radix Salviae Mihiorrhiae.water extract for 24 h were determined by flow cytometry（FCM）.Results：Radix Salviae Miltiorrhiae.water extract inhibited the proliferation of A549 cells in a dose-dependent manner.And the best proliferation was ＇after being treated for 48 h;Morphological changes were dramatical under optical microscopy;In experiment group appeared yellow or chrysoidine fluorescence,cont racted,beaded and crescent and the number of apoptotic cells increased;FCM analysis showed that cells were induced apoptosis dose-dependently.Conclusion：Radix Salviae Miltiorrhiae.water extract has significant antitumor activity in vitro.The potential mechanism of anti-proliferation effect caused by Radix Salviae Miltiorrhiae.water extract may be cell apoptosis.