姜黄素抑制宫颈癌细胞株增殖和诱导凋亡的体外研究

The Study of Cervical Cancer SiHa cell Inhibition and Apoptosis Induced by Curcume

目的：探讨中药姜黄素（Cur）对子宫颈癌SiHa细胞株的增殖抑制和诱导凋亡的作用。方法：以不同浓度的姜黄素（10umol／L、20umol／L、30umol／L），分12h、24h、48h、72h4个时间点处理SiHa细胞，光镜观察细胞形态变化；用四甲基偶氮唑蓝（MTT）法测定细胞增殖抑制率；用透射电子显微镜及琼脂糖凝胶电泳检测凋亡的发生。结果：姜黄素可抑制SiHa细胞增殖，作用呈明显的时效和量效关系，差异有非常显著性（P〈0.01）；姜黄素可诱导SiHa细胞凋亡，透射电子显微镜下可见凋亡细胞；琼脂糖凝胶电泳出现细胞凋亡典型的DNA“梯状”条带。结论：姜黄素体外对SiHa细胞具有增殖抑制作用和促进凋亡作用。

Objective： To investigate the SiHa cells growth inhibition and Apoptosis induced by Curcumin. Methods： SiHa cells were treated with various concentrations （10 umol/L,20 umol/L,30 umol/L ） of Curcume for different periods（12h, 24h, 48h, 72h）. Cell changing was observed by microscope; cell growth was measured by MTT. Apoptosis was detected by different methods including electron microscope and DNA gel electrophoresis. Results： After treated with different concentrations of Curcumin, the cell growth of SiHa was suppressed significantly with showed dose-and time-dependent manner increase in proliferative inhibition rate（P〈0.01）. The Curcumin can induce SiHa cells apoptosis, SiHa cells apoptosis can be observed by electron microscope and agarose gel electrophoresis revealed that cell DNA fragment exhibited characteristic＂ DNA ladder＂. Conclusion： This study suggests that cureumin could inhibit the proliferation and increase apoptosis in human SiHa cells.