丹参多酚盐治疗多脏器功能不全的实验研究

The Therapeutic Effects of Salvianolate Injection Against Multiple Organ Dysfunction Syndrome

目的:探讨丹参多酚盐体内治疗多脏器功能不全的作用。方法:健康大耳白兔24只,随机分为正常对照组、内毒素组、丹参多酚盐干预组,每组8只。内毒素组经耳缘静脉静推LPS(1ml/kg),12h后再由耳缘静脉注射一次LPS(1ml/kg),复制SIRS/MODS动物模型;对照组耳缘静脉静推等量生理盐水;丹参多酚盐干预组于0h,12h经耳缘静脉推注LPS(1ml/kg)并于第一次注入内毒素后6h,12h,18h静推丹参多酚盐(50mg/kg)。于24h时间点测定各组动物血清肿瘤坏死因子-α(TNF-α)、白细胞介素-1(IL-1)、白细胞介素-6(IL-6)、血小板活化因子(PAF)。光镜下观察各组动物脏器病理学变化。结果:丹参多酚盐干预组与内毒素组相比,IL-1、IL-6、PAF、TNF-α均较内毒素组有不同程度降低。丹参多酚盐干预组脏器损伤组织学改变较内毒素组明显减轻。结论:丹参多酚盐具有治疗多脏器功能不全的作用,为防治MODS提供了实验依据。

Objective： To observe the therapeutic effect of Salvianolate injection against multiple organ dysfunction syndrome（MODS）. Methods： 24 rabbits were randomly divided into three groups： control group （n= 8）, MODS group （n= 8）, treatment group （n= 8）. The MODS group were given endotoxin （1mL · kg^-1） twice at 0, 12 hours timepoint; the control group were given injection of normal saline （1mL · kg^-1）, and the treatment group were given Salvianolate injection （300mg · kg ^-1） 3 times at 6,12, 18 hours timepoints besides the injection of endotoxin （1 mL · kg^-1）. The serum concentration of TNF-α, IL-1, IL-6, PAF were measured at 24 hours time points. The pathological changes were observed under microscope. Results： TNF-α, 1L-1, IL-6, PAF concentrations of the treatment group were significant different from those of control group and injury group. Pathology showed Salvianolate intervention group reduced lung and renal injury. Conclusion： Salvianolate injection has therapeutic effect against multiple organ dysfunction syndrome.